{"id":89542,"date":"2026-01-28T13:31:52","date_gmt":"2026-01-28T13:31:52","guid":{"rendered":"https:\/\/www.boccard.com\/fermentation-en-biotechnologie-e-coli-pour-la-production-de-proteine-recombinante-a-lechelle-rd-et-pilote\/"},"modified":"2026-06-19T11:11:45","modified_gmt":"2026-06-19T11:11:45","slug":"fermentation-en-biotechnologie-e-coli-pour-la-production-de-proteine-recombinante-a-lechelle-rd-et-pilote","status":"publish","type":"post","link":"https:\/\/www.boccard.com\/fr\/fermentation-en-biotechnologie-e-coli-pour-la-production-de-proteine-recombinante-a-lechelle-rd-et-pilote\/","title":{"rendered":"Fermentation en biotechnologie : E. coli pour la production de prot\u00e9ine recombinante \u00e0 l\u2019\u00e9chelle R&#038;D et pilote"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"89542\" class=\"elementor elementor-89542 elementor-85810\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section 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M7.8,12.5V7.3c0-1.7,1.4-3.1,3.1-3.1H32c1.7,0,3.1,1.4,3.1,3.1l0,0v5.2c0,1.7-1.4,3.1-3.1,3.1  H10.9C9.2,15.6,7.8,14.2,7.8,12.5C7.8,12.5,7.8,12.5,7.8,12.5z M40.5,56.7c0,1.7-1.4,3.1-3.1,3.1c0,0,0,0,0,0H5.5  c-1.7,0-3.1-1.4-3.1-3.1l0,0V37.8c0-3.6,2-8.7,4.5-11l5.1-5v-2.6h18.9v2.6l5.2,5c2.7,3,4.3,6.9,4.5,11V56.7z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tPHARMA<br \/>&amp; BIOTECH\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-5ad2b619 bg-over elementor-hidden-mobile\" data-id=\"5ad2b619\" data-element_type=\"column\" data-e-type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-59001e41 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"59001e41\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-33345bb1\" data-id=\"33345bb1\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6a0b33c3 elementor-widget elementor-widget-text-editor\" data-id=\"6a0b33c3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div id=\"text_block-296-1724\" class=\"ct-text-block\"> <\/div><p><strong>L\u2019utilisation<em> d\u2019Escherichia coli<\/em> pour la production<\/strong> de prot\u00e9ines recombinantes a jou\u00e9 un r\u00f4le majeur dans la biotechnologie moderne depuis des d\u00e9cennies et est d\u00e9sormais syst\u00e9matiquement standardis\u00e9e en bior\u00e9acteurs afin d\u2019assurer la r\u00e9p\u00e9tabilit\u00e9 et le scale-up. C\u2019est le cas de cet institut europ\u00e9en qui a investi dans une ligne de propagation allant de petits bior\u00e9acteurs en<strong> verre de 5 litres \u00e0 un second bior\u00e9acteur de 30 L, puis \u00e0 150 L<\/strong> pour la production principale. Le proc\u00e9d\u00e9 int\u00e8gre des analyses en ligne pH\/DO (oxyg\u00e8ne dissous)\/glucose, NEP\/SEP (nettoyage\/st\u00e9rilisation en place). Le downsteam process comprend plusieurs \u00e9tapes dont la centrifugation l\u2019extraction par ultrasons et ultrafiltration, et une cuve d\u2019inactivation des d\u00e9chets (contr\u00f4l\u00e9e en pH\/temp\u00e9rature), le tout dans un espace restreint de type bureau. L\u2019objectif : un contr\u00f4le reproductible en amont et un transfert ma\u00eetris\u00e9 vers le downstream process.    <em>D\u00e9couvrez comment Boccard a accompagn\u00e9 cette entreprise dans la r\u00e9alisation de ses objectifs !<\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-38fa0fd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"38fa0fd\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-756b356\" data-id=\"756b356\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0eaa65d elementor-widget elementor-widget-heading\" data-id=\"0eaa65d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\"><b>Quelle est l\u2019histoire derri\u00e8re E. coli et la fermentation des prot\u00e9ines recombinantes\u202f?<\/b><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b5cf95f elementor-widget elementor-widget-text-editor\" data-id=\"b5cf95f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Historiquement, les m\u00e9thodes d\u2019ADN recombinant avec <em>E<\/em>. <em>coli<\/em> ont men\u00e9 \u00e0<strong> l\u2019insuline humaine<\/strong>, le premier m\u00e9dicament recombinant \u00e9tant approuv\u00e9 par la FDA en 1982. Ce fut une \u00e9tape majeure ouvrant l\u2019\u00e8re moderne des biopharmaceutiques et prouvant que des bact\u00e9ries modifi\u00e9es pouvaient produire en toute s\u00e9curit\u00e9 des prot\u00e9ines humaines \u00e0 grande \u00e9chelle. <\/p><p>Aujourd\u2019hui, <strong><em>E<\/em>. <em>coli<\/em> g\u00e9n\u00e9tiquement modifi\u00e9e<\/strong> est utilis\u00e9e pour produire l\u2019hormone de croissance humaine, l\u2019interf\u00e9ron\u2011\u03b12b (pour l\u2019h\u00e9patite chronique et certains cancers), l\u2019interleukine\u20112 (pour l\u2019immunoth\u00e9rapie du carcinome r\u00e9nal m\u00e9tastatique et du m\u00e9lanome) ainsi qu\u2019un groupe d\u2019enzymes th\u00e9rapeutiques ou industrielles\u202f: L\u2011asparaginase pour la leuc\u00e9mie lymphoblastique aigu\u00eb, divers biocatalyseurs pour la fabrication.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t<div class=\"elementor-element elementor-element-d327f84 e-flex e-con-boxed e-con e-parent\" data-id=\"d327f84\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-cbe66e5 e-con-full e-flex e-con e-child\" data-id=\"cbe66e5\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e782853 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e782853\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-beb7812\" data-id=\"beb7812\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7e30b2c elementor-widget elementor-widget-heading\" data-id=\"7e30b2c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\"><b>Que recouvre la \u201cfermentation\u201d ici : Upstream vs. Downstream\u202f?<\/b><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a4111bf elementor-widget elementor-widget-text-editor\" data-id=\"a4111bf\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fermentation upstream : <\/strong>\u00e9tape de multiplication cellulaire<em><em> E. coli<\/em><\/em> g\u00e9n\u00e9tiquement modifi\u00e9e puis d\u2019induction de l\u2019insuline , et production dans des bior\u00e9acteurs \u00e0 cuve agit\u00e9e avec contr\u00f4le du pH\/DO (oxyg\u00e8ne dissous)\/temp\u00e9rature, a\u00e9ration, agitation et alimentation, plus strat\u00e9gie d\u2019induction. <\/p><p><strong>Downstream<\/strong> comprend la phase d\u2019extraction et purification de l\u2019insuline<\/p><ul><li>Centrifugation pour r\u00e9colter le produit par s\u00e9paration m\u00e9canique de densit\u00e9<\/li><li>Sonication pour la lyse ou cassure des parois cellulaires, pour les produits intracellulaires\/p\u00e9riplasmiques, c\u2019est\u2011\u00e0\u2011dire des prot\u00e9ines synth\u00e9tis\u00e9es \u00e0 l\u2019int\u00e9rieur des cellules de la biomasse<\/li><li>Ultrafiltration pour concentrer et clarifier avant la purification chromatographique.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-9ecfdad elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9ecfdad\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-ab3e52c\" data-id=\"ab3e52c\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-14ee2c7 elementor-widget elementor-widget-heading\" data-id=\"14ee2c7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\"><b>Quels sont les d\u00e9fis du passage de bior\u00e9acteurs en verre \u00e0 des bior\u00e9acteurs industriels\u202f?<\/b><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c3d2ffe elementor-widget elementor-widget-text-editor\" data-id=\"c3d2ffe\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Le passage des \u00e9quipements en verre aux bior\u00e9acteurs de 30 et 150\u202fL n\u2019est pas simplement une question de \u201cplus grand volume\u201d. En effet, <strong>l\u2019hydrodynamique, le transfert de masse\/chaleur et l\u2019assurance de la st\u00e9rilit\u00e9 <\/strong>changent fondamentalement. <\/p><p style=\"text-align: left;\"><span style=\"color: #34bef1;\"><strong>La m\u00e9thode de st\u00e9rilisation \u00e9volue de l\u2019autoclave au SEP\u202f:<\/strong><\/span><\/p><p style=\"text-align: left;\">\u00c0 5\u202fL, les r\u00e9cipients et lignes, surtout en verre, sont g\u00e9n\u00e9ralement <strong>st\u00e9rilis\u00e9s en autoclave (ex situ)<\/strong>. \u00c0 30\u202fL et 150\u202fL, les r\u00e9acteurs en inox sont <strong><a href=\"https:\/\/www.boccard.com\/fr\/solutions\/stations-nep-et-sep\/\">st\u00e9rilis\u00e9s sur place<\/a><\/strong> par vapeur via la double enveloppe et la tuyauterie associ\u00e9e. Ce changement n\u00e9cessite des s\u00e9quences automatis\u00e9es, un contr\u00f4le pr\u00e9cis de la vapeur, des temps de maintien valid\u00e9s et une couverture compl\u00e8te des sondes, filtres et connexions pour garantir l\u2019asepsie de tout le flux.  <\/p><p><span style=\"color: #34bef1;\"><strong>Perte de l\u2019approche visuelle en cours de fermentation\u202f:<\/strong><\/span><\/p><p>Dans le verre, les op\u00e9rateurs se fient \u00e0 des <strong>indices visuels directs<\/strong>. En inox, on <strong>d\u00e9pend davantage de l\u2019instrumentation<\/strong> et des donn\u00e9es\u202f: pH, oxyg\u00e8ne dissous et analyses de glucose, temp\u00e9rature, pression et flux de gaz sont essentiels pour la mont\u00e9e en semence, l\u2019induction et la r\u00e9colte. Les superpositions de lots, alarmes et tendances historiques remplacent l\u2019observation directe et aident \u00e0 maintenir la r\u00e9p\u00e9tabilit\u00e9 d\u2019un lot \u00e0 l\u2019autre.  <\/p><p><span style=\"color: #34bef1;\"><strong>La gestion thermique devient un probl\u00e8me de contr\u00f4le, pas seulement un r\u00e9glage\u202f:<\/strong><\/span><\/p><p>Les r\u00e9acteurs plus grands en <a href=\"https:\/\/www.boccard.com\/fr\/solutions\/cuves-inox-et-metaux-nobles\/\">inox<\/a> ont une i<strong>nertie thermique<\/strong> plus \u00e9lev\u00e9e. Les circuits \u00e0 double enveloppe, la capacit\u00e9 des utilit\u00e9s et les boucles de contr\u00f4le ajust\u00e9es doivent g\u00e9rer la chaleur m\u00e9tabolique durant la croissance et les chocs thermiques de l\u2019induction, du NEP et du SEP sans d\u00e9passement. Le but est un <strong>profil thermique stable<\/strong> qui prot\u00e8ge la qualit\u00e9 du produit et garde les temps de cycle sous contr\u00f4le.  <\/p><p><span style=\"color: #34bef1;\"><strong>Le transfert et le m\u00e9lange des gaz doivent \u00eatre repens\u00e9s :<\/strong><\/span><\/p><p>Le type d\u2019agitateur, la g\u00e9om\u00e9trie du diffuseur, le m\u00e9lange gazeux, la contre-pression et la strat\u00e9gie antimousse sont dimensionn\u00e9s pour optimiser le <strong>taux de transfert d\u2019oxyg\u00e8ne (TTO)<\/strong> et l\u2019homog\u00e9n\u00e9isation \u00e0 grande \u00e9chelle. Une <strong><a href=\"https:\/\/www.boccard.com\/fr\/solutions\/systemes-dagitation\/\">expertise en ing\u00e9nierie du m\u00e9lange<\/a><\/strong> est n\u00e9cessaire pour atteindre l\u2019objectif d\u2019une absorption d\u2019oxyg\u00e8ne identique, \u00e9viter les gradients et g\u00e9rer le cisaillement afin que les cellules soient expos\u00e9es au m\u00eame environnement \u00e0 150 L qu\u2019\u00e0 5 L.<\/p><p><span style=\"color: #34bef1;\"><strong>Le scale-up impose des contraintes de nettoyabilit\u00e9 et de conformit\u00e9 :<\/strong><\/span><\/p><p>Des <strong>configurations<\/strong> avec syst\u00e8me de vidange, des s\u00e9quences NEP valid\u00e9es et des transferts en circuit ferm\u00e9 r\u00e9duisent les risques de contamination. En pr\u00e9sence d\u2019organismes g\u00e9n\u00e9tiquement modifi\u00e9s ou en cas de pr\u00e9occupations<strong> li\u00e9es \u00e0 la bios\u00e9curit\u00e9<\/strong>, une cuve d\u2019inactivation des d\u00e9chets d\u00e9di\u00e9e, avec contr\u00f4le de la temp\u00e9rature et du pH, constitue une barri\u00e8re v\u00e9rifiable avant rejet. <\/p><p><span style=\"color: #34bef1;\"><strong>L\u2019automatisation et l\u2019ergonomie sont essentielles dans les espaces r\u00e9els :<\/strong><\/span><\/p><p>Passer d\u2019une <strong>paillasse \u00e0 une salle de type bureau<\/strong> implique la conception d\u2019une ligne <strong>compacte<\/strong> tout en garantissant un acc\u00e8s s\u00e9curis\u00e9, des canalisations claires et une utilisation ais\u00e9e. Les recettes automatis\u00e9es simplifient les t\u00e2ches routini\u00e8res et r\u00e9duisent la variabilit\u00e9 li\u00e9e \u00e0 l\u2019op\u00e9rateur, tout en pr\u00e9servant la flexibilit\u00e9 pour la R&#038;D. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t<div class=\"elementor-element elementor-element-1a97fd8 e-flex e-con-boxed e-con e-parent\" data-id=\"1a97fd8\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b1102e0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b1102e0\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-a139ecc\" data-id=\"a139ecc\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8a37e57 elementor-widget elementor-widget-heading\" data-id=\"8a37e57\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\"><b>Comment Boccard a-t-il accompagn\u00e9 le client dans l\u2019am\u00e9lioration de son processus de prot\u00e9ines recombinantes\u202f?<\/b><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b11de34 elementor-widget elementor-widget-text-editor\" data-id=\"b11de34\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Boccard a con\u00e7u et livr\u00e9 un bior\u00e9acteur de<strong> 30 L (pH\/DO\/glucose) alimentant un bior\u00e9acteur principal de 150 L ; int\u00e9gr\u00e9 centrifugeuse, sonication et ultrafiltration pour une r\u00e9cup\u00e9ration compacte \u00e0 l\u2019\u00e9chelle pilote\u202f; et a install\u00e9 un syst\u00e8me d\u2019inactivation des d\u00e9chets (temp\u00e9rature + pH).<\/strong> Le skid et les utilit\u00e9s ont \u00e9t\u00e9 adapt\u00e9s \u00e0 un petit espace de type bureau, avec NEP\/SEP et automatisation pour des lots r\u00e9p\u00e9tables.<\/p><p>En r\u00e9sum\u00e9, Boccard a d\u00fb construire une ligne de fermentation<strong> conforme aux normes environnementales et de s\u00e9curit\u00e9 li\u00e9es<\/strong> \u00e0 l\u2019inactivation des d\u00e9chets, <strong>respectant l\u2019installation ergonomique<\/strong> malgr\u00e9 des locaux contraints ainsi qu\u2019une automatisation polyvalente et <strong>facile \u00e0 prendre en main<\/strong>. Ce sont les attentes du client que Boccard a r\u00e9ussi \u00e0 satisfaire. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4c4d4bb elementor-widget elementor-widget-image\" data-id=\"4c4d4bb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1083\" height=\"771\" src=\"https:\/\/www.boccard.com\/wp-content\/uploads\/2026\/01\/Tekinbio_Case_Study_2_3_EN_01_26.png\" class=\"attachment-full size-full wp-image-89535\" alt=\"\" srcset=\"https:\/\/www.boccard.com\/wp-content\/uploads\/2026\/01\/Tekinbio_Case_Study_2_3_EN_01_26.png 1083w, https:\/\/www.boccard.com\/wp-content\/uploads\/2026\/01\/Tekinbio_Case_Study_2_3_EN_01_26-300x214.png 300w, https:\/\/www.boccard.com\/wp-content\/uploads\/2026\/01\/Tekinbio_Case_Study_2_3_EN_01_26-1024x729.png 1024w, https:\/\/www.boccard.com\/wp-content\/uploads\/2026\/01\/Tekinbio_Case_Study_2_3_EN_01_26-768x547.png 768w\" sizes=\"(max-width: 1083px) 100vw, 1083px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-04d4d84 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"04d4d84\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5060845\" data-id=\"5060845\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8a2f5b5 elementor-widget elementor-widget-heading\" data-id=\"8a2f5b5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\"><b>Quels bior\u00e9acteurs Boccard soutiennent la fermentation et le scale-up au-del\u00e0 du pilote\u202f?<\/b><\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-de470d1 elementor-widget elementor-widget-text-editor\" data-id=\"de470d1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div id=\"text_block-296-1724\" class=\"ct-text-block\"><p>Les m\u00eames piliers sont standard dans <a href=\"https:\/\/www.boccard.com\/fr\/solutions\/tekinbio-fermenteur-pour-de-micro-organismes\/\"><strong>TEKINBIO\u2122, le bior\u00e9acteur Boccard pour la fermentation et le scale-up,<\/strong><\/a> avec <strong><a href=\"https:\/\/www.boccard.com\/fr\/solutions\/aide-a-la-performance-track-advance\/\">Track Advance comme socle digital pour le suivi en temps r\u00e9el, la gestion des recettes, et la comparaison lot \u00e0 lot <\/a><\/strong>\u2014un pont pratique et auditable du pilote \u00e0 l\u2019industriel.<\/p><p>TEKINBIO\u2122 int\u00e8gre :<\/p><ul><li><strong>A\u00e9ration optimis\u00e9e :<\/strong> g\u00e9om\u00e9trie du sparger, transfert d\u2019O\u2082, gestion de la pression du ciel de cuve<\/li><li><strong>M\u00e9lange parfait :<\/strong> homog\u00e9n\u00e9isation valid\u00e9e par CFD et ma\u00eetrise du cisaillement<\/li><li><strong>Contr\u00f4le thermique pr\u00e9cis<\/strong><\/li><li><strong>Conception hygi\u00e9nique avec NEP\/SEP<\/strong><\/li><li><a href=\"https:\/\/www.boccard.com\/fr\/solutions\/aide-a-la-performance-track-advance\/\">Track Advance <\/a>pour les recettes, la comparaison des lots et la conformit\u00e9 r\u00e9glementaire<\/li><\/ul><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b637be1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b637be1\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-db882d4\" data-id=\"db882d4\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-044c6e7 elementor-cta--skin-classic elementor-animated-content elementor-bg-transform elementor-bg-transform-zoom-in elementor-widget elementor-widget-call-to-action\" data-id=\"044c6e7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"call-to-action.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-cta\">\n\t\t\t\t\t<div class=\"elementor-cta__bg-wrapper\">\n\t\t\t\t<div class=\"elementor-cta__bg elementor-bg\" style=\"background-image: url(https:\/\/www.boccard.com\/wp-content\/uploads\/2025\/12\/Tekinbio_Page_Web_7_EN_12_2025-1024x727.jpg);\" role=\"img\" aria-label=\"tekinbio\"><\/div>\n\t\t\t\t<div class=\"elementor-cta__bg-overlay\"><\/div>\n\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-cta__content\">\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<h2 class=\"elementor-cta__title elementor-cta__content-item elementor-content-item\">\n\t\t\t\t\t\tEn savoir plus sur nos solutions pour<br \/>la fermentation et Pharma &amp; Biotech ? \t\t\t\t\t<\/h2>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-cta__button-wrapper elementor-cta__content-item elementor-content-item \">\n\t\t\t\t\t<a class=\"elementor-cta__button elementor-button elementor-size-\" href=\"https:\/\/www.boccard.com\/fr\/contact\/\">\n\t\t\t\t\t\tContactez-nous\t\t\t\t\t<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-415afe6 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"415afe6\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-2edeb26\" data-id=\"2edeb26\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-bcd2852 elementor-widget elementor-widget-heading\" data-id=\"bcd2852\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\"><b>Points cl\u00e9s<\/b><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-77fb1bf barre elementor-widget elementor-widget-heading\" data-id=\"77fb1bf\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Ligne \u00e0 deux r\u00e9acteurs : 30 L semence (pH\/DO\/glucose) \u2192 150 L principal ; CIP\/SIP ; modules aval compacts (centrifugeuse, sonication, UF).<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-335f23d barre elementor-widget elementor-widget-heading\" data-id=\"335f23d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Inactivation des d\u00e9chets : cuve d\u00e9di\u00e9e avec contr\u00f4le temp\u00e9rature &amp; pH avant rejet \u00e0 l\u2019\u00e9gout.<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e8a6440 barre elementor-widget elementor-widget-heading\" data-id=\"e8a6440\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Contraintes d\u2019espace r\u00e9solues : int\u00e9gration dans un espace de type bureau avec utilit\u00e9s et ergonomie adapt\u00e9es.<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-410d017 barre elementor-widget elementor-widget-heading\" data-id=\"410d017\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Expertise du scale-up : documentation du train de semence, conception OTR\/m\u00e9lange, capacit\u00e9 thermique, asepsie et tra\u00e7abilit\u00e9 des recettes.<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-58a81d5 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"58a81d5\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-cb6d518\" data-id=\"cb6d518\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-86b2451 elementor-widget elementor-widget-heading\" data-id=\"86b2451\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\"><br><b>Scope : \u00e9quipements et services fournis <br \/>Ing\u00e9nierie &amp; pr\u00e9fabrication<\/b><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-8f93e77 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"8f93e77\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-edbf430\" data-id=\"edbf430\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-858bf2a elementor-widget elementor-widget-text-editor\" data-id=\"858bf2a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li><strong><a href=\"https:\/\/www.boccard.com\/fr\/solutions\/ingenierie-travaux-neufs\/\">Scope \u2014 \u00c9quipements et services fournis :<\/a><\/strong>Ing\u00e9nierie &#038; pr\u00e9fabrication train de bior\u00e9acteurs 30 \u202f L + 150 \u202f L avec analyses en ligne, unit\u00e9s downstream int\u00e9gr\u00e9es (centrifugeuse, sonication, UF), armoires de contr\u00f4le, et tuyauterie modulaire\u2014con\u00e7u pour espaces compacts et facilit\u00e9 d\u2019installation.<\/li><li><strong><a href=\"https:\/\/www.boccard.com\/fr\/solutions\/stations-nep-et-sep\/\">Utilit\u00e9s &#038; conception hygi\u00e9nique NEP\/SEP,<\/a><\/strong> vapeur, gaz et raccordements eau de proc\u00e9d\u00e9\u202f; disposition hygi\u00e9nique pour l\u2019asepsie et la r\u00e9p\u00e9tabilit\u00e9\u202f; utilit\u00e9s thermiques (g\u00e9n\u00e9rateur de vapeur\/groupe froid).<\/li><li><strong><a href=\"https:\/\/www.boccard.com\/fr\/solutions\/genie-des-procedes\/\">Activation du proc\u00e9d\u00e9 (campagnes prot\u00e9ines recombinantes) <\/a><\/strong>fermentation semence-principal (ex. 5 \u202f L verre \u2192 30 \u202f L \u2192 150 \u202f L), strat\u00e9gie d\u2019induction, et fen\u00eatres op\u00e9ratoires (pH\/DO\/temp\/OTR, agitation), avec documentation pour des comparaisons de lots robustes et transfert aval.<\/li><li><strong>Vers l\u2019industrialisation : <a href=\"https:\/\/www.boccard.com\/fr\/solutions\/tekinbio-fermenteur-pour-de-micro-organismes\/\">TEKINBIO\u2122<\/a> + <a href=\"https:\/\/www.boccard.com\/fr\/solutions\/aide-a-la-performance-track-advance\/\">Track Advance<\/a><\/strong> Transf\u00e9rer les expertises pilote dans <a href=\"https:\/\/www.boccard.com\/fr\/solutions\/tekinbio-fermenteur-pour-de-micro-organismes\/\">TEKINBIO\u2122<\/a> , associ\u00e9 \u00e0 <a href=\"https:\/\/www.boccard.com\/fr\/solutions\/aide-a-la-performance-track-advance\/\">Track Advance<\/a> pour le suivi en temps r\u00e9el , la gestion des recettes , la comparaison des lots , et la tra\u00e7abilit\u00e9 r\u00e9glementaire \u2014un chemin \u00e9prouv\u00e9 du pilote \u00e0 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elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3dbdff30\" data-id=\"3dbdff30\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-26d61c56 elementor-hidden-mobile elementor-widget elementor-widget-spacer\" data-id=\"26d61c56\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>D\u00e9couvrez comment la mont\u00e9e en \u00e9chelle de la fermentation de prot\u00e9ines recombinantes d\u2019E. coli passe d\u2019un bior\u00e9acteur en verre de 5 L \u00e0 une cuve de semence de 30 L puis \u00e0 un bior\u00e9acteur principal de 150 L, avec des analyses en ligne du pH, de l\u2019oxyg\u00e8ne dissous (DO) et du glucose, ainsi que des syst\u00e8mes CIP\/SIP et une centrifugeuse compacte, suivis de sonication et d\u2019ultrafiltration. Un r\u00e9servoir d\u2019inactivation des effluents, contr\u00f4l\u00e9 en temp\u00e9rature et en pH, permet une \u00e9limination s\u00fbre dans un espace restreint. Gr\u00e2ce \u00e0 TEKINBIO\u2122 et Track Advance\u00ae, les \u00e9quipes b\u00e9n\u00e9ficient d\u2019un contr\u00f4le renforc\u00e9 et d\u2019une mont\u00e9e en \u00e9chelle tra\u00e7able pour une industrialisation fiable  <\/p>\n","protected":false},"author":29,"featured_media":89536,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[69,39],"tags":[],"class_list":["post-89542","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-pharma-biotech","category-realisations"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Fermentation en biotechnologie : E. coli pour la production de prot\u00e9ine recombinante \u00e0 l\u2019\u00e9chelle R&amp;D et pilote - Boccard<\/title>\n<meta name=\"description\" content=\"E. coli fermentation from glass to pilot: recombinant protein 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